Composite
Part:BBa_K2350017:Design
Designed by: YI-CHIEN LIN Group: iGEM17_NYMU-Taipei (2017-10-22)
J23118-B0034-NrtA-B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1240
Illegal BamHI site found at 610 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
we want the NrtA be produced continuously,so we choose a moderate constitutive promoter.
Source
Synechocystis sp. PCC 6803 genomic DNA